Coding

Part:BBa_K1982002:Design

Designed by: Zexu Li   Group: iGEM16_NEU-China   (2016-09-11)


Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BglII site found at 870
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 56
    Illegal SapI.rc site found at 164


Design Notes

mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg


Source

The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.

References